This sample-solvent parameter is addressed as the refractive index increment, dn/dc. The sample itself also plays an important role: the higher the polarizability of the sample (compared to the solvent), the higher the signal intensity. The higher the molar mass and the concentration, the higher the measured signal intensities. Their signal response depends on both the concentration and the molar mass. Light scattering detectors are molar mass sensitive detectors. These detectors should never be confused with evaporative light scattering detectors (ELSDs) (2), which measure scattered light of analyte droplets after mobile phase evaporation. The light scattering (LS) detector models differ mainly in the position where the scattered light is measured and in the number of simultaneously acquired signals. This instalment of Tips & Tricks discusses column issues when working with light scattering detectors.ĭetectors to measure the intensity of scattered light in solution are very popular additions to gel permeation chromatography/size-exclusion chromatography (GPC/SEC) instruments because they add true value (1). However, the complexity of the system also increases. The addition of a light scattering detector dramatically increases the capabilities of gel permeation chromatography/size-exclusion chromatography (GPC/SEC) analysis.
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